4 edition of Fluorescence Studies on Biological Membranes (Subcellular Biochemistry) found in the catalog.
Fluorescence Studies on Biological Membranes (Subcellular Biochemistry)
Herwig J. Hilderson
October 31, 1988
Written in English
|The Physical Object|
|Number of Pages||465|
Using a pulsed, tunable dye laser details of the fluorescence behavior of single, intact human red blood cells have been studied. The high sensitivity of the laser excited fluorescence allows the detection of potential-dependent uptake and release of dye added to the suspension of living erythrocytes. The membrane potential of the erythrocytes was changed by means of different . Fluorescence-quenching and resonance energy transfer studies of lipid microdomains in model and biological membranes (Review). Molecular Membrane Biology , 23 (1), DOI: /
Currently, studying membrane proteins in vitro is a daunting task as they often require isolation protocols that are challenging to optimize and are non-generalizable. State-of-the-art strategies for preparing samples for fluorescence studies of membrane proteins involve the solubilization of the target membrane protein with detergent, site-selective labeling, and finally reconstitution . The book is organized in three parts, the first of which deals with membrane probes and model membranes. The second part describes the use of advanced quantitative and high-resolution techniques to explore the properties of biological membranes, illustrating the key progress made regarding membrane organization, dynamics and : Springer Berlin Heidelberg.
Multi scale fluorescence studies: the spinning disk confocal fluorescence lifetime imaging microscopy & single molecule nano-metronome & single molecule study of tetramethyrhodamine-DNA interaction. PhD in Biophysics and Computational Biology, University of Illinois at Urbana-Champaign, Cell membranes organize and regulate a vital share of biological signaling. Fluorescence has become the main analytical tool with which to study such systems, and novel fluorescence techniques drive biological discovery at ever-smaller size and time scales.
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Therefore, we have brought together an international group of experts in order to report on and review advances in fluorescence studies on biological membranes, thereby highlighting subcellular aspects.
The first chapters present a critical evaluation of the current applications of dynamic and steady-state fluorescence techniques. “The book covers all major applications of fluorescence to membrane studies. The book is addressed to scientists and PhD students using fluorescence methods to study biological membranes and membrane proteins.
This is a highly interdisciplinary field, and the book will therefore be of interest to biophysicists, physical chemists, and. Fluorescent Methods to Study Biological Membranes (Springer Series on Fluorescence Book 13) - Kindle edition by Mely, Yves, Duportail, Guy. Download it once and read it on your Kindle device, PC, phones or tablets.
Use features like bookmarks, note taking and highlighting while reading Fluorescent Methods to Study Biological Membranes (Springer Series on Fluorescence Book Manufacturer: Springer. Get this from a library. Fluorescence Studies on Biological Membranes. [H J Hilderson] -- As stated by its first editor, Dr.
D.B. Roodyn, the primary goal of the series Subcellular Biochemistry is to achieve an integrated view of the cell by bringing together results from a wide range of. Therefore, we have brought together an international group of experts in order to report on and review advances in fluorescence studies on biological membranes, thereby highlighting subcellular Subsequent chapters dis- cuss more specific applications in cells, biological membranes, and their con- stituents (lipids, proteins).
Fluorescence Studies on Biological Membranes. Editors: Hilderson, Herwig J. (Ed.) Free Preview. Buy this book eB49 € price for Spain (gross) Buy eBook ISBN ; Digitally watermarked, DRM-free; Included format: PDF; ebooks can be Brand: Springer US.
Fluorescence techniques have become powerful and widely used tools for studies of biochemical and biophysical processes occurring in biological membranes. Various fluorescence methods have played and continue to play key roles in modern membrane science, so that there have been several focused reviews on this topic.
The book is organized in three parts, the first of which deals with membrane probes and model membranes. The second part describes the use of advanced quantitative and high-resolution techniques to explore the properties of biological membranes, illustrating the key progress made regarding membrane organization, dynamics and interactions.
As briefly stated in a previous section on the fluid mosaic model of biological membranes, proteins and phospholipids diffuse both laterally and, to a lesser extent transversely, through the entire span of a membrane. This sort of behavior can be characterized by fluorescence microscopy.
Fluorescence-quenching and resonance energy transfer studies of lipid microdomains in model and biological membranes (Review) JOHN R. SILVIUS1 & IVAN ROBERT NABI2 1Department of Biochemistry, McGill University, Montre´al, Que´bec, and 2Department of Cellular and Physiological Sciences, University of British Columbia, Vancouver, BC, Canada.
Bagatolli, LLAURDAN fluorescence properties in membranes: a journey from the fluorometer to the microscope. in Y Mely & G Duportail (eds), Fluorescent Methods to study biological membranes. Springer, Heidelberg, Springer Series on Fluorescence, no.
13, vol. Fluorescent Methods to study Biological membranes, pp. The book is organized in three parts, the first of which deals with membrane probes and model membranes. “The book covers all major applications of fluorescence to membrane studies.
The book is addressed to scientists and PhD students using fluorescence methods to study biological membranes and membrane proteins. This is a Author: Yves Mely.
The ability to record fluorescence counts from a single, defined focal volume for a long period of time (1–10 s) is an advantage of FCS.
This enables the study of a broad range of processes, from fast photophysical dynamics such as fluorophore blinking (1–10 μs) to the slower diffusion of molecules in solution or the cytoplasm. Fluorescent lipid probes have proven to be valuable tools in membrane studies (see Ref.
1 for review). Because the determination of depth-dependent parameters of bilayers can benefit the understanding of membranous structures (), sets of probes bearing the same fluorophore at different distances from the bilayer surface are potentially quite useful.
Fluorescence techniques have become powerful and widely used tools for studies of biochemical and biophysical processes occurring in biological membranes. Various fluorescence methods have played. Using fluorescence for studies of biological membranes: a review Alexander Kyrychenko Institute of Chemistry and School of Chemistry, V.
Karazin Kharkiv National University, 4 Svobody Square. Fluorescence measurements have been also used to monitor the binding and transport of FAs in model and biological membranes. In particular, the authors showed the positioning of the FAs in the PL bilayers, their partition between the bilayers and the aqueous buffer, and the presence of the FA carboxyl groups at the outer leaflet of membranes.
Available from UMI in association with The British Library. Requires signed TDF. A time-correlated single photon counting spectrometer has been constructed and developed for fluorescence emission anisotropy studies of biological membranes.
The instrument was used to study the fluorescence emission anisotropy of blood membrane samples taken from patients suffering. Biological membranes are preferentially composed of lipids and proteins, and it is assumed that mainly the proteins are responsible for their functional properties.
Nevertheless, during the last years, the contribution of the plain lipid matrix and its physico-chemical parameters to membrane functionality has been shown to be of high relevance. Fluorescence micrographs that capture the sizes, shapes, and distributions of liquid domains in model membranes have provided high standards of evidence to prove (and disprove) theories of how micron-scale domains form and grow.
Some theories about smaller domains have remained untested, partly because experimental methods of identifying. I. Fluorescence polarization studies of fatty acid-altered EL4 tumor cell membranes. Biochimica et Biophysica Acta (BBA) - Biomembranes(1), DOI: /(81) John W.
Allis, Barbara L. Sinha. Fluorescence depolarization studies of red cell membrane fluidity.Membranes, an international, peer-reviewed Open Access journal. Dear Colleagues, Membranes is also open to submission (research articles, short communications, and review articles) on biological membranes.
We are launching a Topical Collection on "Feature Papers in Membranes in Life sciences”, which will include all the papers in the section “Membranes in .Fluorescence spectra, decay times and emission anisotropy are capable of providing important information regarding the interaction of probes with biological macromolecules.
Combinations of different fluorescence parameters can provide information about heterogeneity of liposomes or biological membranes and about th.